Heterologous expression of diadenylate cyclase in the form of inclusion bodies with enzymatic activity

Using the DNA recombination technique, a new bacterial strain Escherichia coli DAC-22 was derived, whose cells are able to carry out heterologous expression of Bacillus thuringiensis diadenylate cyclase – enzyme catalyzing reaction adenosine-5′-triphosphate (ATP) transformation into cyclic 3′,5′-diadenylate (cyclo-di-AMP). To derive strain, E. “Rosetta (DE3) pLysS” were originally used as recipients plasmid pET42a+ with inserted gene disA encoding B. thuringiensis. The recombinant produce localized predominantly (by 90 %) in fraction catalytically active inclusion bodies. productivity respect structurally arranged bodies 720 units/l cultural fluid. formed by newly engineered intended for use technology producing pharmacologically promising cyclo-di-AMP.